Table 1.

The immunization process of BALB/c mice

Group	Immunization time a
	First time	Second time	Third time
PBS	100 μl	100 μl	100 μl
pEGFP-C1	100 μg	100 μg	100 μg
α-GalCer	100 μl PBS	100 μl PBS	2 μg α-GalCerb
pEGFP-C1-SAG5D	100 μg	100 μg	100 μg
α-GalCer/pEGFP-C1-SAG5D	100 μg	100 μg	100 μg
		pEGFP-C1-SAG5D	pEGFP-C1-SAG5D/2 μg α-GalCer

^aAll mice were injected three times at two week interval.

^b2 μg α-GalCer was diluted with 100 μl PBS before use.