Figure 5.
Effect of VCG on BMDC presentation of chlamydial antigen to immune and naïve CD4+ T cells. BMDC (2 × 105 cells/well) pulsed for 24 h with UV-EB in the presence or absence of VCG were cultured with purified immune (A) or naïve (B) CD4+ T cells (2 × 105 cells/well) for 48 h. Control cultures contained BMDCs and purified T cells without antigen (DC alone; DC+CM). At the end of the incubation period, supernatants were harvested and assayed for Th1 and Th2 cytokines, using the Bio-Plex cytokine assay kit in combination with the Bio-Plex Manager software. The concentration of the cytokine in each sample was obtained by extrapolation from a standard calibration curve generated simultaneously. Data were calculated as the mean values (± S.D.) for triplicate cultures for each experiment. The results are from two independent experiments. Significant differences between DCs alone and treated DCs and between DCs pulsed with UV-EB with and without VCG are indicated by two asterisks (P <0.01) and an asterisk (P <0.05) respectively.