Figure 7.

Memory antibody and Th1/Th2 cytokine responses elicited 24 days postchallenge. Groups of mice were adoptively immunized and challenged intravaginally as described in the materials and methods section. About 24 days postchallenge, serum, vaginal lavage and spleens were collected. The amount of memory antibodies (A) elicited in genital lavage and serum was assessed by an antibody ELISA. Results generated simultaneously with a standard curve display data sets corresponding to absorbance values as mean concentrations (ng/ml) ± SD of triplicate cultures for each experiment. The results are from two independent experiments. *Statistically significant (p < 0.05) differences between DC + EB and DC + EB + VCG groups. Also, purified splenic T cells were restimulated in vitro with UV-irradiated chlamydial EBs and IFN-γ and IL-4 production were assessed as a measure of Thl and Th2 responses, respectively. The amount of cytokine contained in supernatants of culture-stimulated cells was measured by single cytokine ELISA. The concentration of the cytokine in each sample was obtained by extrapolation from a standard calibration curve generated simultaneously. Data were calculated as the mean values (± S.D.) for triplicate cultures for each experiment (B). The results are from two independent experiments. *Statistically significant (p < 0.05) differences between DC + EB and DC + EB + VCG groups.