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. 2014 Dec 31;15:584. doi: 10.1186/s12865-014-0056-x

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© Eko et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of VCG on reduction of IFU in adoptively immunized mice. Immunized mice were challenged intravaginally with 5 x 10⁴ IFU of live chlamydiae 24 days after the last immunization. One week prior to challenge, mice were administered Depo Provera to stabilize the estrous cycle and facilitate a productive infection. Infections were monitored by cervicovaginal swabbing of individual animals every 3 days and Chlamydia was isolated from swabs in tissue culture and enumerated. The data show the mean recoverable IFUs ± S.D. The experiment was repeated to contain 6 mice per group.