Table 1.
Average response to LPS from young and old cultures as measured by increase in expression of immune associated genes as compared to hour 0 post-LPS
| Gene symbol | Gene name | Fold change* | |
|---|---|---|---|
| Hour 2 | Hour 8 | ||
| Transcription and activation pathways | |||
| BIRC3 | Baculoviral IAP repeat-containing protein 3 | 6.1 | 4.1 |
| NFKBIA | Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha | 36.1 | 10 |
| NFKBIZ | Nuclear factor of kappa-B inhibitor zeta | 23.8 | 4.1 |
| NFKB2 | Nuclear factor of κ light polypeptide gene enhancer in B-cells 2 | 2 | 2.1 |
| Cytokines and chemokines, growth factors | |||
| CCL2 | Chemokine (C-C motif) ligand 2 | 28.5 | 26.7 |
| CCL5 | RANTES | 35.4 | 48.4 |
| CCL20 | Chemokine (C-C motif) ligand 20 | 2034 | 1162.3 |
| CSF2 | Colony stimulating factor 2 | 97 | 6.1 |
| CXCL1 | Chemokine (C-X-C motif) ligand 1 | 2.5 | - - |
| CXCL2 | Chemokine (C-X-C motif) ligand 2 | 43.8 | 27.1 |
| CXCL5 | Chemokine (C-X-C motif) ligand 5 | 43.8 | 27.1 |
| IFNB | Interferon beta precursor | 2 | 2 |
| IL1A | Interleukin 1, alpha | 86.0 | 23.5 |
| IL-6 | Interleukin 6 | 83 | 131.6 |
| IL-8 | Interleukin 8 | 123.7 | 273.7 |
| SAA3 | Serum Amyloid A 3 | 505.6 | 2498.3 |
| TNF-a | Tumor Necrosis Factor, alpha | 125.2 | - - |
| TNFSF13B | Tumor necrosis factor (ligand) superfamily, member 13 (BAFF) | - - | 4.6 |
| Type I IFN-related genes | |||
| IFI44 | Similar to Interferon-induced protein 44 | - - | 15.2 |
| IFIH1 | Interferon induced with helicase C domain 1 | - - | 15.1 |
| ISG15 | ubiquitin-like modifier | 6.2 | 63.2 |
| MX2 | Myxovirus (influenza virus) resistance 2 | - - | 121.2 |
| OAS1 | 2′-5′-oligoadenylate synthetase 1 | - - | 24.6 |
| OAS2 | 2′-5′-oligoadenylate synthetase 2 | 2.8 | 26.1 |
| TNFSF10 | Tumor necrosis factor ligand superfamily member 10 (TRAIL) | - - | 15.8 |
*Data obtained by RNA-Seq and presented as fold induction of the indicated gene at either 2 or 8 hours post-LPS in comparison to expression levels at 0 hours post-LPS. All fold changes shown are FDR < 0.05; FC > 2; and CPM >1. - - indicates FDR > 0.05, FC < 2, or CPM < 1.