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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Mol Cancer Res. 2014 Sep 3;13(1):50–62. doi: 10.1158/1541-7786.MCR-14-0440

Figure 7. Induction of HO-1 is sufficient to rescue Tsc2-null cells from chelerythrine chloride-induced death.

Figure 7

A) Tsc2+/+ and Tsc2−/− MEFs were treated with chelerythrine chloride (2 uM, 2 h). Hmox1, SOD2, and TXN1 levels were measured by quantitative RT-PCR and normalized to beta actin. B) Tsc2+/+ and Tsc2−/− null MEFs were treated with chelerythrine chloride (2µM) or vehicle alone. Cells were harvested after 6 hours, the nuclear and cytoplasmic fractions were isolated from these cells and the expression of Bach-1 was quantified by western blot analysis. Expression of SP-1 and GAPDH were used to assess the purity of the nuclear and cytoplasmic fractions, respectively. C) Densitometry was used to quantitate the results of three independent experiments. *p<0.05, two-sided T-Test. D) Tsc2+/+ and Tsc2−/− MEFs were treated for 20 hours with DMSO or Hemin (10 uM) in replicate cultures; chelerythrine chloride (2 uM, 2 h) was added for the final 2 hr to the indicated wells. Phase contrast images (4X) show rescue of chelerythrine chloride-induced death by Hemin in the Tsc2−/− cells. E) Immunoblot showing induction of HO-1 by Hemin.