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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Mol Cancer Res. 2014 Aug 28;13(1):78–85. doi: 10.1158/1541-7786.MCR-14-0334

Figure 2.

Figure 2

Notch 3 is required for survival of IGROV-1 cells. (A) Cell count analysis by trypan blue exclusion showed significantly fewer cells at 96h between 0h siCtrl-48h siCtrl and 0h siNotch3-48h siNotch3. (B) Treatment of siNotch3 IGROV-1 cells with the pan-caspase inhibitor Z-VAD-FMK showed a significant reduction of apoptotic cells compared with vehicle treated siNotch3 cells. There was a significant (p < 0.01) increase in trypan blue positive cells in siNotch3 DMSO treated cells as compared with siControl DMSO treated cells and a non-significant reduction in siControl cells treated with Z-VAD-FMK. These relationships are not shown for clarity of the figure. (C) siNotch3 cells express cleaved PARP whereas siControl cells do not.