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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Mol Cancer Res. 2014 Aug 28;13(1):78–85. doi: 10.1158/1541-7786.MCR-14-0334

Figure 5.

Figure 5

Notch3 signaling promotes Akt and Erk regulation of Bim. Notch3 signaling induces phosphorylation of Akt Ser473 and Erk 1/2 in both adherent and non-adherent conditions. (A) The reduction of pAkt and pErk 1/2 result in an increase in Bim expression. Inhibition of PI3K is sufficient to reduce both Akt and Erk 1/2 activation. (B) Treatment of siCtrl and siNotch3 cells with the PI3K inhibitor LY294002 (50μM) for 1h in adherent conditions is sufficient to inhibit phosphorylation of Erk 1/2 comparable to vehicle treated siNotch3 cells.