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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Mol Cancer Res. 2014 Aug 28;13(1):78–85. doi: 10.1158/1541-7786.MCR-14-0334

Figure 6.

Figure 6

Notch3 is required for proper expression of col4α2. Both IGROV-1 and OVCAR-3 siNotch3 cells express significantly less col4α2 mRNA (A and B) than their control counterparts (** p = < 0.01, *** = p < 0.005, **** = p < 0.001). Col4α2 mRNA expression is not significantly altered in siNotch1 cells. (C) Protein-level expression of the col4α2 chain is reduced in IGROV-1 siNotch3 cells as compared with siControl cell by immunoblot. (D) Exogenous collagen IV rescues Notch3 knockdown cells (** = p < 0.01, *** = p < 0.005). IGROV-1 cells 24h post-transfection with Notch3 siRNA show significantly less cell death when plated on exogenous human collagen type IV for 24h than siNotch3 cells plated in human collagen Type I or control wells (p < 0.01).