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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Kidney Int. 2014 Aug 6;87(2):332–342. doi: 10.1038/ki.2014.270

Figure 1.

Figure 1

Interferons induce APOL1 expression and appearance of additional transcript variants. Normalized expression of APOL1 (to 18S subunit) in (A) Human Coronary Artery Endothelial Cells (HCAEC) or (B) podocytes after stimulation with α (100U/ml), β (100U/ml), or γ(10ng/ml) interferon. Values are mean fold increase in APOL1 mRNA +/− s.e.m for a minimum of 3 experiments. (C) Under basal conditions in HCAEC, only APOL1 transcript variant (tv) 1 was detected by PCR. After stimulation with Interferon γ, tv2 and tv4 were also detected at time points indicated. N-terminal amino acid sequences encoded by the transcript variants are shown at right. Red caret indicates predicted signal sequence cleavage site present in tv1, but absent from tv4. The site in tv2 may be too far from the N-terminus to promote cleavage. (D) Western blot of APOL1 protein in whole cell lysate 24 hours after stimulation with polyI:C (10µg/ml) or interferons (α-100U/ml, β-100U/ml, or γ-10ng/ml) in endothelial cells and podocytes. (E) APOL1 staining was not observed in untreated (control) endothelial cells. APOL1 staining (green) is strong after 24 hours of interferon γ treatment and can be abolished by APOL1 siRNA knockdown. Nuclei are stained by DAPI (blue).