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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Kidney Int. 2014 Aug 6;87(2):332–342. doi: 10.1038/ki.2014.270

Figure 2.

Figure 2

The TLR3 agonist PolyI:C upregulates APOL1 expression. Normalized APOL1 expression (to 18S subunit) measured by real-time RT-PCR in (A) HCAEC or (B) podocytes after 24 hours of stimulation by the indicated agonists for the specified TLRs. Values are fold increase in APOL1 mRNA/18S mRNA expression for 3 experiments +/− s.e.m. TLR1/2: Pam3cSK4 (triacylated lipoprotein), 1µg/ml; TLR2: HKLM (derived from heat-killed Listeria), 108 cells/ml; TLR3: poly(I:C), low (0.2–1kb) or high (1.5–8kb) molecular weight, 10µg/ml; TLR4: Lipopolysaccharide (LPS), 1µg/ml, TLR5: Flagellin, 1µg/ml; TLR6/2: FSL1 (diacylated lipoprotein), 1µg/ml; TLR7: Imiquimod, 2µg/ml; TLR8: ssRNA40/Lyovec, 2µg/ml; TLR9: ODN compounds (unmethylated CpG synthetic oligos), 1µM.