Western blot analysis for Axin2 in canine melanoma cell lines (CML-10C2, UCDK9M2, and UCDK9M3) treated with 5 µM BIO or equivalent volume of DMSO vehicle control for 72 h. Quantification of blots was performed using the Gel Logic 100 Imaging System (Kodak). Numerical values, i.e. relative expression (RE), represent the density of Axin2 in BIO-treated cells relative to its corresponding DMSO-treated cells of the same cell line, adjusted by the densities of the corresponding loading controls (actin).