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. Author manuscript; available in PMC: 2015 Aug 21.
Published in final edited form as: Nanoscale. 2014 Jul 8;6(16):9742–9751. doi: 10.1039/c4nr01510j

Figure 3. Cellular uptake and intracellular location of Tf-NP-siRNA in MV4–11 cells.

Figure 3

Figure 3

Figure 3

(A) Flow cytometry analysis on expression levels of TfR (also known as CD71) on the surface of AML cells. Cells were surface stained with PE-labeled anti-TfR (anti-CD71) monoclonal antibodies (BD Biosciences, San Jose, CA) for 30min on ice, followed by flow cytometry analysis. (B) Cellular uptake of FAM-labeled Tf-NP-siRNA by flow cytometry. (C) Intracellular localization of Tf-NP-siRNA 4 h after transfection by confocal microscopy.