Table 1.

Real Time PCR primers and conditions

Species	Genome	Target Gene	Forward Primer Seq 5′-3′	Reverse Primer Seq 5′-3′	Amplicon (bps)	Annealing temperature	Reference
C. elegans	mt	nd-1	AGC GTC ATT TAT TGG GAA GAA GAC	AAG CTT GTG CTA ATC CCA TAA ATG T	75	62	[14]
	nuc	Cox-4	GCC GAC TGG AAG AAC TTG TC	GCG GAG ATC ACC TTC CAG TA	164	62	this chapter
H. sapiens	mt	tRNA-Leu(UUR)	CAC CCA AGA ACA GGG TTT GT	TGG CCA TGG GTA TGT TGT TA	107	62	[21]
	nuc>	B2-microglobulin	TGC TGT CTC CAT GTT TGA TGT ATC T	TCT CTG CTC CCC ACC TCT AAG T	86	62