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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Nat Genet. 2008 Dec 11;41(3):354–358. doi: 10.1038/ng.306

Figure 4.

Figure 4

The missense mutations in NKB results in severe loss of agonist function at the NK3R A) Representative Ca2+-responses of 3 wt-TACR3 transfected cells to application of different NKB and NKB-M90T doses as indicated. B) NKB dose responses for NKB (black squares), and NKB-M90T (red circles). Peak [Ca2+]-responses recorded as in (A) in 5 dishes (n=113 cells) were averaged over 30s and the data was fit with the logistic function [Ca2+] = (A1-A2)/(1+([NKB]/EC50))+A2 using origin software (Microcal). The resulting fit parameters were: NKB: A1=40±8 nM, A2=242±11 nM, EC50=5.8±1.5 nM; NKB-M90T: A1=38±4 nM, A2=242 nM (fixed), EC50=52±6 nM. Error bars represent 1SE; responses to similar doses of the two peptides were compared using a paired t-test; i p=3*10−12, ii p=3*10−20, iii p=6*10−7.