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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Dec 20;1851(3):261–272. doi: 10.1016/j.bbalip.2014.12.007

Figure 2. Characterization of PLD inhibitors FIPI and NFOT.

Figure 2

A) Western blot analysis of PLD2 purification. B) Determination of linearity for PLD2 activity and inhibition. C) Increasing concentrations of purified recombinant PLD2 was assayed in the absence or presence of FIPI or NFOT. D) Shown is the Michaelis-Menten kinetics plot of FIPI and NFOT respective to the control. E) Shown is the Lineweaver-Burke plot of FIPI and NFOT respective to the control. A second line could be derived in NFOT experimental points if one considers high concentration of substrate (>10 mM). This is indicated by a dashed line in panel E.