Figure 1. Expression of the vitamin D intracrine system and antibacterial cathelicidin in monocytes cultured in serum from HIV+ and HIV- patients.
Human monocytes from normal healthy donors were cultured in serum (10%) from age-matched HIV+ or HIV- donors in the presence or absence of a toll-like receptor 2/1 ligand (TLR2/1L), PAM3CysK for 24 hrs. Total RNA from these cultures was then used to assess expression of: mRNA for CYP27B1, VDR, CYP24A1, and CAMP. Data are shown as fold-change in TLR2/1L-treated cells relative to vehicle-treated controls cells for each gene ± SE. * = HIV+ statistically different from HIV- subjects, p<0.05; ** p<0.01.