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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Reprod Toxicol. 2014 Aug 1;49:86–100. doi: 10.1016/j.reprotox.2014.07.074

Figure 5. ROCK, RhoA and eNOS protein expression in rat aortic endothelial cells (RAEC) with 12 hours exposure to C60/PVP or PVP.

Figure 5

RAEC were treated in vitro with C60/PVP at 1–10 µg/cm2 concentration range for 12 hours. RhoA (A), ROCK1 (B), ROCK2 (C) and eNOS (D) expression was assessed by In-cell Western assay. The expression of the target proteins were normalized to the cell number and then normalized to the untreated control (considered as 100%). * indicates p < 0.05 when compared between the means (calculated using 3–5 independent experiments) of treatment groups using a two tailed t test