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. Author manuscript; available in PMC: 2016 Feb 11.
Published in final edited form as: Vaccine. 2015 Jan 3;33(7):869–878. doi: 10.1016/j.vaccine.2014.12.056

Table 1.

Q-PCR primer sequence

Gene
Forward primer
Reverse primer
IFNα 5’CCTGATGAAGGAGGACTCCATT3’ 5’AAAAAGGTGAGCTGGCATACG3’
IFNβ 5’GTCTCCTCCAAATTGCTCTC3’ 5’ACAGGAGCTTCTGACACTGA3’
IL-1β 5’ACGAATCTCCGACCACCACT3’ 5’CCATGGCCACAACAACTGAC3’
IL-6 5’GCAACACCAGGAGCAGCC3’ 5’AACTCCTTCTCCACAAGCGC3’
IL-12 5’TGGAGTGCCAGGAGGACAGT3’ 5’TCTTGGGTGGGTCAGGTTTG3’
TNFα 5’GCCCAGGCAGTCAGATCATCT3’ 5’TTGAGGGTTTGCTACAACATGG3’
A20 5’TCCTCAGGCTTTGTATTTGAGC3’ 5’TGTGTATCGGTGCATGGTTTTA3’
IκBα 5’CTCCGAGACTTTCGAGGAAATAC3’ 5’GCCATTGTAGTTGGTAGCCTTCA3’
GAPDH 5’CTCAAGATCATCAGCAATGCCT3’ 5’AAGTTGTCATGGATGACCTTGG3’