Table 3.
Gas production of feed stuffs incubated in buffered rumen fluid with different additives (mL g-1 DM).
Treatments |
Incubation times (h)
|
||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2 | 4 | 6 | 8 | 12 | 16 | 24 | 36 | 48 | 72 | 96 | a+b | c (h-1) | |
CTR | 24.83a | 32.84a | 69.10a | 95.61a | 119.54a | 142.60a | 193.93a | 224.64a | 271.60a | 287.88a | 309.88a | 309.08a | 0.031cd |
MON | 19.93c | 24.14b | 57.80b | 83.98b | 106.93b | 128.98b | 159.22b | 192.19b | 215.10ab | 226.27c | 249.45c | 243.50d | 0.032c |
GAR 1 | 23.44ab | 29.26a | 63.78ab | 87.23b | 115.30ab | 136.56ab | 183.22a | 208.82ab | 224.21b | 257.62b | 279.17b | 272.30c | 0.04a |
GAR 2 | 24.42a | 32.59a | 70.04a | 100.44a | 122.50a | 143.54a | 189.31a | 223.13a | 249.00ab | 286.37a | 310.59a | 309.18a | 0.03b |
GAR 3 | 16.00d | 25.26b | 61.28b | 95.75a | 124.04a | 145.30a | 188.63a | 221.12a | 243.80ab | 280.58a | 299.24ab | 300.00b | 0.04a |
EO 1 | 20.44bc | 21.37b | 21.73c | 24.64c | 30.67c | 61.69c | 92.80c | 108.22c | 123.50ab | 135.61d | 147.39d | 156.61e | 0.03c |
EO 2 | 20.44bc | 22.12b | 23.24c | 27.22c | 29.59c | 30.79d | 53.38d | 85.28d | 100.80b | 107.26e | 113.48e | 145.85f | 0.01e |
EO 3 | 21.78abc | 23.60b | 24.51c | 25.54c | 26.98c | 27.80d | 43.36d | 65.97e | 86.11b | 104.44e | 113.00e | 132.72g | 0.02e |
SEM | 1.82 | 2.25 | 3.62 | 4.13 | 4.89 | 5.33 | 7.23 | 10.68 | 10.84 | 11.61 | 12.21 | 0.277 | 0.00 |
DM = dry matter, CTR = control, MON = monensin 7.5 µg mL-1, GAR1 = raw garlic bulb 5 mg mL-1, GAR2 = raw garlic bulb 10 mg mL-1, GAR3 = raw garlic bulb 15 mg mL-1, EO1 = garlic essential oil 33 µg mL-1, EO2 = garlic essential oil 66µg mL-1, EO3 = garlic essential oil 100 µg mL-1, a+b = potential of gas production mL g-1 DM, c = rate constant of gas production during incubation mL h-1
The means within a same column without common letter(s) differ significantly (P < 0.05).