Table 1.
Primer sequences and predicted lengths of PCR amplification products.15
| Gene | Primer |
Oligonucleotide
sequence (5-3) |
Fragment
size (pb) |
|---|---|---|---|
| stx1 | SLT1-F | TGTAACTGGAAAGGTGGAGTATACA | 210 |
| SLT1-R | GCTATTCTGAGTCAACGAAAAATAAC | ||
| stx2 | SLTII-F | GTTTTTCTTCGGTATCCTATTCC | 484 |
| SLTII-R | GATGCATCTCTGGTCATTGTATTAC | ||
| ehlyA | AE22 | ATTACCATCCACACAGACGGT | 397 |
| AE20-2 | ACAGCGTGGTTGGATCAACCT | ||
| eaeA | MFS1-F | ACGATGTGGTTTATTCTGGA | 166 |
| MFS1-R | CTTCACGTCACCATACATAT | ||
| fli | FLICH7-F | GCGCTGTCGAGTTCTATCGAGC | 625 |
| FLICH7-R | CAACGGTGACTTTATCGCCATTCC |