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. 2014 Oct 29;308(3):C249–C263. doi: 10.1152/ajpcell.00265.2014

Fig. 4.

Fig. 4.

Glucose and palmitate decrease AMPK activity in HAECs. A: AMPK activity was determined by SAMS peptide assay (n = 8), expressed in radiolabeled cpm/mg protein. B: relative change in the ratio of phosphorylated (Thr172) AMPK (p-AMPK α1/α2)/total AMPK (t-AMPK α1) protein is shown (n = 6) with a representative Western blot below the graph. C: densitometric analysis of the ratio of phosphorylated (Thr172) AMPK (p-AMPK α1/α2) to total AMPK (t-AMPK α1) protein indicates that aminoimidazole-4-carboxamide-1-β-4-ribofuranoside (AICAR) increases AMPK phosphorylation (n ≥ 5). A representative Western blot is shown below the graph. D: densitometric analysis of the ratio of phosphorylated (Ser79) acetyl-CoA carboxylase (p-ACC)/total ACC (t-ACC) protein (a target of active AMPK) indicates activation of AMPK by AICAR (n ≥ 8). A representative Western blot is shown below the graph. For A and B, *P < 0.05 compared with control treatment, and for C and D, *P < 0.05 for the effect of AICAR (two-way ANOVA).