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Reactive oxygen species (ROS) production in AC5Tg myocytes. A: representative images showing MitoSOX Red-loaded WT and AC5Tg cardiomyocytes under control conditions and after exposure to 1 μM isoproterenol for 40 min. B: summary of MitoSOX Red fluorescence in WT and AC5Tg cardiomyocytes in the absence (vehicle) or presence of 1 μM isoproterenol (Iso). Cell number is indicated above each bar (from 3–5 mice each). *P < 0.05, **P < 0.01.
