Fig. 4.
Effect of monocarboxylate transport inhibitors on acetate-induced Isc increases. Luminal acetate-induced ΔIsc was measured in the presence or absence of the SMCT1 inhibitor ibuprofen (Ibu, A), the MCT inhibitors α-cyano-4-hydroxycinnamic acid (4-CHCA, B), or UK-5099 (C). Ibu was added into the luminal whereas 4-CHCA was added into the serosal bathing solution. UK-5099 followed by 30 mM acetate was added to the luminal (M) or serosal (S) bath. Pretreatment with 1 mM ibuprofen, 4-CHCA, or serosal UK-5099 significantly decreased the response to luminal acetate; n = 3−5. *P < 0.05 vs. the vehicle group.