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Sequencing of bisulfite-treated DNA encoding miR-143 and miR-145 in proximal and distal colon. After bisulfite conversion and PCR amplification, DNA sequencing was performed in 4 subjects for the indicated loci in proximal and distal colon. Each line represents a single clone. Black boxes, methylated CpG sites; gray boxes, unmethylated CpG sites. Ten representative clones for each sample are included.
