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. 2014 Nov 12;308(3):F244–F251. doi: 10.1152/ajprenal.00596.2013

Fig. 3.

Fig. 3.

NOS1 is necessary for ET-1-mediated inhibition of ENaC. Representative current traces from cell-attached patches made on the apical membrane of principal cells in split-open murine collecting ducts from flox control (A; n = 6 mice, n = 10 patches) and CDNOS1KO (B) mice (n = 6 mice, n = 16 patches) before (top) and after (bottom) treatment with 20 nM ET-1. The closed state is denoted with a dashed line. Inward current is downward. The holding potential for these patches was −Vp = −60 mV. Summary graphs of ENaC Po in control (C) and CDNOS1KO (D) mice before (vehicle) and after ET-1. Data are from experiments identical to that in A–B. *Significantly decreased compared with before ET-1. **Significantly greater than flox control under identical conditions.