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PCR Primer Sequences
| Primer name | Gene | Sequence 5′–3′ | Tm | nt # | Amplicon size (bp) |
|---|---|---|---|---|---|
| hGAPDH_FW | GAPDH | TCCTCCACCTTTGACGCTG | 60 | 19 | 101 |
| hGAPDH_Rw | ACCACCCTGTTGCTGTAGCC | 62 | 20 | ||
| hMLH1_gI7-E8_FW | MLH1 | ttatcagCAAGGAGAGACAGTAGC | 59 | 24 | 108 |
| hMLH1_gE8-I9_Rv | cgacatacCGACTAACAGCATTT | 60 | 23 | ||
| hPTEN_gE1_FW | PTEN | GCCAAGTCCAGAGCCATT | 60 | 18 | 225 |
| hPTEN_gI1_Rv | aagagagtgacagaaaggtaaagagg | 60 | 26 | ||
| hTP53_gE3-I3_FW | TP53 | CTGgtaaggacaagggttgg | 59 | 20 | 316 |
| hTP53_gE4_Rv | CTGGGAAGGGACAGAAGATG | 60 | 20 |
