Fig. 1.

Box whisker plots of IL-12 (A) and IFN-γ (B) levels in spleen homogenates of mice (n=6) after administration of booster doses with BCG, Ag85B, and Ag85 peptides. Twenty-eight days after last vaccination, mice from respective groups were sacrificed and their spleens were isolated, homogenized and stimulated with PPD. IL-12, interleukin 12; IFN-γ, interferon γ; BCG, Bacillus Calmette-Guerin; Ag85B, antigen 85B; PPD, purified protein derivative. Data was analyzed with the Student t-test (^***p<0.001, BCG/BCG vs. BCG and control; ^***p<0.001, Ag85B vs. BCG and control; ^***p<0.001, peptide vs. all group). Error bars indicate the standard error of the mean; data are representative of 2 independent experiments.