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IgG levels were high in intramuscular (IM) administered unadsorbed vaccine (0.5 µg/mL) of 1.0-mL dose volume, compared to the reduced (0.5 µg/mL) intradermal (ID) immunization of only 0.1-mL volume, which indicated the role of using both adjuvant adsorbed vaccine and in a nano-size particles.
