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Agarose gel electrophoresis of enzyme-digested products (a) and amplicons from the multiplex PCR assay (b). Lanes M, DL2000 ladder; lanes 1, CBS2030 (C. guilliermondii); lanes 2, Y-27403 (C. fermentati); lanes 3, Y-17323 (C. palmioleophila); lanes 4, CBS1795 (C. famata); lanes 5, CBS796 (D. fabryi); lanes 6, CBS2334 (D. nepalensis). Bands of <100 bp in the RFLP analysis are not shown.
