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. 2014 Sep;52(9):3190–3195. doi: 10.1128/JCM.01297-14

TABLE 1.

Primers used in this study

Primer Sequence (5′ to 3′) Position Purpose
ITS1 TCCGTAGGTGAACCTGCGG 18S rDNA ITS sequencing
ITS4 TCCTCCGCTTATTGATATGC 26S rDNA ITS sequencing
IGS1F TGTAAGCAGTAGAGTAGCCTTGTTG 26S rDNA IGS1 sequencing
IGS1R AGACCGAGTAGTGTAGTGGGAGAC 5S rDNA IGS1 sequencing
ITS2F GATGTATTAGGTTTATCCAACTCGT ITS2 rDNA PCR-RFLP assay
26SR TCATTTCAACCCCAATACCTC 26S rDNA PCR-RFLP assay
DG5F GCCCTCCTTCTTAGCTCGTAWGTAT RPL18 gene Multiplex PCR assay
DG5R GGCAGATGACCTTGTTGAATGG RPL18 gene Multiplex PCR assay
GuF TGCTATATCTTTGGCTCAGCG IGS1 rDNA Multiplex PCR assay
GuR GTCGTCTAGCATTGGTTTTGACT IGS1 rDNA Multiplex PCR assay
PalF GCGGCGAATTGTTATTTAATACT ITS1 rDNA Multiplex PCR assay
PalR GTGAATGCACTTCTCAGCGTC ITS2 rDNA Multiplex PCR assay