TABLE 1.
Primer | Sequence (5′ to 3′) | Position | Purpose |
---|---|---|---|
ITS1 | TCCGTAGGTGAACCTGCGG | 18S rDNA | ITS sequencing |
ITS4 | TCCTCCGCTTATTGATATGC | 26S rDNA | ITS sequencing |
IGS1F | TGTAAGCAGTAGAGTAGCCTTGTTG | 26S rDNA | IGS1 sequencing |
IGS1R | AGACCGAGTAGTGTAGTGGGAGAC | 5S rDNA | IGS1 sequencing |
ITS2F | GATGTATTAGGTTTATCCAACTCGT | ITS2 rDNA | PCR-RFLP assay |
26SR | TCATTTCAACCCCAATACCTC | 26S rDNA | PCR-RFLP assay |
DG5F | GCCCTCCTTCTTAGCTCGTAWGTAT | RPL18 gene | Multiplex PCR assay |
DG5R | GGCAGATGACCTTGTTGAATGG | RPL18 gene | Multiplex PCR assay |
GuF | TGCTATATCTTTGGCTCAGCG | IGS1 rDNA | Multiplex PCR assay |
GuR | GTCGTCTAGCATTGGTTTTGACT | IGS1 rDNA | Multiplex PCR assay |
PalF | GCGGCGAATTGTTATTTAATACT | ITS1 rDNA | Multiplex PCR assay |
PalR | GTGAATGCACTTCTCAGCGTC | ITS2 rDNA | Multiplex PCR assay |