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Comparison of sensitivities of C. difficile brucella broth with thioglycolic acid and l-cystine (CDBB-TC) versus C. difficile brucella broth (CDBB) in the laboratory. Ten-microliter aliquots of serial dilutions of spores of two test strains were applied to polyester swabs that were inoculated into 15 ml of CDBB-TC or prereduced CDBB and incubated for 72 h at 37°C. Specimens with a color change from red to yellow were cultured to confirm the growth of C. difficile.
