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Formulation of Clostridium difficile brucella broth with thioglycolic acid and l-cysteine
| Ingredient | Quantity/liter |
|---|---|
| Brucella broth | 28.0 g |
| Vitamin K1 solution (1 mg/ml) | 1.0 ml |
| Hemin solution (5 mg/ml) | 1.0 ml |
| Sodium bicarbonate solution (20 mg/ml) | 5.0 ml |
| d-Mannitol | 6.0 g |
| Neutral red solution (1%) | 5.0 ml |
| Sodium taurocholate | 0.5 g |
| Lysozyme | 5.0 mg |
| d-Cycloserine | 500.0 mg |
| Cefoxitin | 16.0 mg |
| Agar | 1.0 g |
| Thioglycolic acid (mercaptoacetic acid) | 1.0 g |
| l-Cystine | 1.0 g |
