FIG 4.

Clinical strains analyzed in the multiplexed nanowell AST. Shown is a heatmap representation of antibiotic susceptibility of clinical strains ARD144 (A), ARD145 (B), and ARD146 (C) against ciprofloxacin (Cipro) and cefotaxime (Cefo) at the indicated concentrations (μg/ml). Positive (strains in M-H II broth) and negative (M-H II broth and M-H II broth plus antibiotics [4 μg/ml of Cipro or 64 μg/ml of Cefo]) controls were included. “Exp no.” refers to the 2 independent experiments, each including 4 rows corresponding to OD₆₀₀s from wells 1 to 4 recorded every 5 min over 12 h. Increased absorbance is depicted as a change from yellow to red along the horizontal axis. Classification of strains at each condition is indicated as susceptible (S) and resistant (R) based on CLSI guidelines.