FIG 2.

Histopathological lesions and BoAstV-CH13 RNA detection in the facial nucleus in the medulla oblongata of a cow with nonsuppurative encephalitis. (A) Affected animals exhibited gliosis and neuronal necrosis. (B) High magnification of the marked area in panel A, showing necrotic neurons. (C to E) In situ hybridization for BoAstV-CH13 with probe A (C), probe B (D), and an unrelated control probe (E). (F and G) Negative-control sections of a healthy cow, analyzed with probe A (F) and probe B (G). Dark-blue labeling, presence of viral RNA. Magnification, ×10 (A and C to G) or ×40 (B).