TABLE 4.
Comparison of the PCR methods
| Specimen IDa | Reported qPCR method (log10 copies/ml of specimen) for segment: |
cvPCR result forb: |
qPCR result (log10 copies/ml of specimen): |
P valuec | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| S | M | L | Mean | Set 1 | Set 2 | NP | GPC | RdRp | Mean | ||
| 057A | 5.02 | 5.75 | 5.60 | 5.46 | + | + | 6.23 | 6.57 | 5.24 | 6.01 | NSd |
| 062A1 | 4.61 | 4.87 | 4.81 | 4.76 | + | + | 5.62 | 6.14 | 5.58 | 5.78 | <0.01 |
| 064A | − | 3.53 | 3.62 | 3.57 | + | + | 4.01 | 4.54 | 3.57 | 4.04 | NS |
| 067A | 5.42 | 5.70 | 5.61 | 5.58 | + | + | 6.75 | 7.13 | 6.72 | 6.86 | <0.001 |
| 078A3 | 3.67 | 4.15 | 4.11 | 3.98 | + | + | 4.69 | 5.23 | 4.46 | 4.79 | NS |
| 082A | 4.76 | 4.89 | 4.89 | 4.85 | + | + | 5.81 | 5.99 | 5.47 | 5.76 | <0.05 |
| 090A | 5.28 | 5.57 | 5.51 | 5.45 | + | + | 6.06 | 6.46 | 6.14 | 6.22 | NS |
| 097A1 | − | 4.86 | 4.43 | 4.64 | + | + | 5.08 | 5.69 | 4.32 | 5.03 | NS |
| 076A | − | − | − | − | − | − | − | − | |||
| 079A | − | − | − | − | − | − | − | − | |||
| 081A | − | − | − | − | − | − | − | − | |||
| 092A | − | − | − | − | − | − | − | − | |||
| 093A | − | − | − | − | − | − | − | − | |||
| 094A1 | − | − | − | − | − | − | − | − | |||
| HB29 (2 × 105 TCID50/ml) | 6.01 | 6.13 | 6.02 | 6.05 | + | + | 6.46 | 6.48 | 6.38 | 6.44 | NS |
a
ID, identification.
b
+, detected; −, not detected.
c
P value was computed using a one-way ANOVA with Bonferroni's multiple-comparison test.
d
NS, not significant.