TABLE 1.

Target genes, specific probe sequences, and primer sequences used in Campylobacter MBiT

No.	Target gene	Left probe oligonucleotide	Right probe oligonucleotide	Product size (nt)a
1	tetO	CAGATACAATGAATTTGGAGCGTCAAAGG	GGAATCACTATCCAGACAGCAGTGACATC	124
2	virB8	GTAAAGGTAGAACAAGCAGGAATGGATATGAG	AGCCGATGAGAATTTATTAAAATCAATTCTCGCAGGCT	142
3	cgtA	CTTTGGATAGCAGGCAATATACTTTCAAGAGAA	GCTTTTAAGGTAATAACTTCATTTTTTACTCGTATAAAAGCCC	160
4	Cj1136	GTTTTTTGCCTCTTTAGCATTTTCTCCAAAAA	ATTCATACAAGTCTTTAAAATACTCTGACACATTTGCC	178
5	panB	GATCGTGACGCTATCTTAAATGCCTCAAG	ATTTATCAAAGAAAGCCACGCAAATGGGGTAAAAGTGGAAG	196
6	maf5	CAGCCAGTTCATAAGCCATATGAGATACACTCATG	CCTGCGTTAATATATCCATAATCGTCTAGCCC	214
7	Cj1135	CGCTTTCATTTTTATTTAAAATTACTCTTGAACCTTGTAAAATTACTTTG	CGTTTTGCAAATTCTAAATGATTTTTTACAAAGTCTTTTTCTAAAATCATATC	232
8	Cj0265	GGATTTACATAACCATCAATTTTTAGCAAAACCCTGTTA	TTTTCGCTTTTTAATACTTCAAAAGGATTTGTAGGTAAAAGTC	250
9	CJE1733	CTTTATGTCTTATTTTGATGATGTATCCGCCTTTG	GCTAAGGTTGATTATGCAAAGCTTTCAAAGGT	268
10	Cj0122	CTAAGCTCTTTTAATAGATATTTTGGAAACAATCCTTTGCAA	ACACTTACCAAAATAAGAGATGAAAGTATTGAAAATGGAAATC	286
11	gmhA2	GTTAATATGCTAGTATCTGTAGTAAGTGCAATACTTGCGATA	CCGGGTCTATCAAAATAAAACCTACTTACAAATTCCC	311
12	flgE2	GTTCTTCTTTGTGGACAGCTACAAATATTACATTTACAC	CACAACCTCCTCAAGCAGCTACGAATGT	338
13	CJE1500	GTTTTGATGATGGTCTAAAAGATCATTACGATTTCG	TTTTCCCTAAACTCTTAGAGCATAAAATTTTTGGATTATTTTTTATACC	365
14	Cj0423	CTGGCTGATAATTTCTTTGCTAAATTTGTTTTTTGG	TTGGACTATTATTGTTTGGCTTGTTTGTTTAATATGGTCT	391
15	wlaN	CCACAATCATCTACTACAATGATTTCTATATCTTTAAAAGTTTGGTTA	ATGCAACTTTCTAATGCTCTAGCAATATATTTTTCCACAT	418
16	cfrA	CCAAAAAAGTAAGTGATAAATGGGAAACTTCTGTAAG	TTTGGATGCTCTTTTAAATGAAAATAAAGATTGGGGTAATACTT	445
17	Cj1321	CATGTAATTTTATCACTTTTATACATCCTCAATCTTTTGTTTCA	AAAGAAGCTAAAATAGGACAAGGGGTTATAGTGTGTGT	473
18	Cj0008	GAAAATAATGTCATAGAATGCCGTAAAAATAGTCCTGAAG	CGTTTGCTGTGTTATCTTTACTTTATCCAAATTTAGATTATAAAAATAATAATTTT	503
	SALSA PCR primers	FAM-GGGTTCCCTAAGGGTTGGA	GTGCCAGCAAGATCCAATCTAGA	NAb

^ant, nucleotides.

^bNA, not applicable. SALSA PCR primers are used to amplify all MLPA probes once the left and right probe oligonucleotides are ligated.