TABLE 3.
Comparison of selected genes detected by microarray among the common MSSA and MRSA bacteremia clones in the cohorta
| Variableb | Data by clonal complex: |
Totalc | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 8 | 5 | 22 | 45 | 30 | 1 | 15 | 88 | 188d | ||
| No. (%) | 69 (35.4) | 26 (13.3) | 20 (10.3) | 18 (9.2) | 15 (7.7) | 14 (7.2) | 11 (5.6) | 11 (5.6) | 11 (5.6) | 195 |
| agr type | ||||||||||
| I | 69 (100) | 0 | 20 (100) | 16 (88.9) | 0 | 0 | 0 | 0 | 11 (100) | 116 |
| II | 0 | 26 (100) | 0 | 0 | 0 | 0 | 11 (100) | 0 | 0 | 37 |
| III | 0 | 0 | 0 | 0 | 15 (100) | 14 (100) | 0 | 11 (100) | 0 | 40 |
| IV | 0 | 0 | 0 | 2 (11.1) | 0 | 0 | 0 | 0 | 0 | 2 |
| Polysaccharide capsule | ||||||||||
| cap5 | 8 (11.6) | 26 (100) | 20 (100) | 0 | 0 | 0 | 0 | 0 | 0 | 54 |
| cap8 | 61 (88.4) | 0 | 0 | 17 (94.4) | 15 (100) | 14 (100) | 11 (100) | 11 (100) | 11 (100) | 140 |
| Antimicrobial resistance | ||||||||||
| blaZ | 66 (95.7) | 22 (84.6) | 19 (95) | 14 (77.8) | 15 (100) | 10 (71.4) | 10 (90.9) | 10 (90.9) | 10 (90.9) | 176 |
| mecA | 59 (85.5) | 6 (23.1) | 18 (90) | 6 (33.3) | 2 (13.3) | 4 (28.6) | 0 | 2 (18.2) | 0 | 97 |
| ermA | 61 (88.4) | 0 | 0 | 1 (5.6) | 5 (33.3) | 0 | 0 | 3 (27.3) | 0 | 70 |
| aacC-aphD | 58 (84.1) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 58 |
| mupR | 1 (1.4) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
| tetK | 34 (49.3) | 0 | 0 | 5 (27.8) | 0 | 0 | 0 | 0 | 0 | 39 |
| fosB | 69 (100) | 26 (100) | 0 | 0 | 15 (100) | 0 | 11 (100) | 1 (9.1) | 0 | 122 |
| qacA | 48 (69.6) | 3 (11.5) | 0 | 0 | 0 | 1 (7.1) | 0 | 5 (45.5) | 0 | 57 |
| Leukocidins or exotoxins | ||||||||||
| PVL | 0 | 0 | 0 | 0 | 2 (13.3) | 1 (7.1) | 0 | 0 | 1 (9.1) | 4 |
| tst | 1 (1.4) | 2 (7.7) | 0 | 0 | 12 (80) | 0 | 0 | 0 | 0 | 15 |
| Enterotoxins | ||||||||||
| sea | 50 (72.5) | 15 (57.7) | 0 | 0 | 9 (60.0) | 10 (71.4) | 0 | 0 | 0 | 84 |
| seb | 1 (1.4) | 2 (7.7) | 2 (10.0) | 0 | 0 | 1 (7.1) | 0 | 1 (9.1) | 1 (9.1) | 8 |
| sec | 0 | 0 | 14 (70.0) | 5 (27.8) | 1 (6.7) | 0 | 0 | 5 (45.5) | 0 | 25 |
| sege | 2 (2.9) | 26 (100) | 20 (100) | 18 (100) | 14 (93.3) | 0 | 0 | 0 | 0 | 80 |
| Exfoliative toxin etA | 0 | 0 | 0 | 0 | 0 | 0 | 1 (9.1) | 2 (18.2) | 0 | 3 |
| Adhesion factors | ||||||||||
| clfA | 59 (85.5) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 59 |
| cna | 61 (88.4) | 0 | 20 (100) | 18 (100) | 14 (93.3) | 14 (100) | 0 | 1 (9.1) | 11 (100) | 139 |
| Miscellaneous | ||||||||||
| ACME locus | 18 (26.1) | 0 | 3 (15.0) | 0 | 0 | 0 | 0 | 0 | 0 | 21 |
| sak | 62 (89.9) | 25 (96.2) | 17 (85.0) | 18 (100) | 15 (100) | 13 (92.9) | 1 (9.1) | 11 (100) | 11 (100) | 173 |
| CHIPS | 4 (5.8) | 17 (65.4) | 17 (85.0) | 18 (100) | 13 (86.7) | 0 | 11 (100) | 0 | 11 (100) | 91 |
| isaB | 8 (11.6) | 26 (100) | 0 | 0 | 1 (6.7) | 14 (100) | 11 (100) | 11 (100) | 11 (100) | 82 |
| splA | 68 (98.6) | 25 (96.2) | 0 | 0 | 0 | 14 (100) | 11 (100) | 11 (100) | 11 (100) | 140 |
| agr dysfunction presentf | 50 (72.5) | 8 (30.8) | 8 (40.0) | 7 (38.9) | 15 (100) | 8 (57.1) | 3 (27.3) | 7 (63.6) | 5 (45.5) | 111 |
a
MSSA, methicillin-susceptible S. aureus; MRSA, methicillin-resistant S. aureus.
b
Data are represented as number (%), unless otherwise indicated. PVL, Panton-Valentine leukocidin; ACME, arginine catabolic mobile element; CHIPS, chemotaxis inhibitory protein of S. aureus.
c
These isolates represent 80.6% of the total tested cohort (195/242 isolates).
d
This does not include the isolate for which clonal complex was unassigned on microarray but clustered with CC188 on phylogenetic analysis.
e
Part of enterotoxin gene cluster (egc).
f
As measured by delta-hemolysin assay.