FIG 1.
Correlation between phylogenetic analysis of GBS isolates based on concatenated alignment of sequenced pbp2x, pbp2b, and pbp1a genes and MLST sequence types. The CTBr PSGBS isolates B1 to B6, PRGBS isolates A1 and A2, and PSGBS isolate B7, characterized in the present study, are shaded. Other isolates, such as PRGBS isolates R1 to R8 and PSGBS isolates N1 to N4, were reported previously (8). Ten PRGBS clinical isolates (strain numbers 1, 2-1, 2-2, 3, 4-1, 4-2, 5, 6, 7, and 8) caused a probable nosocomial transmission in a general hospital (16) and were identical to isolate R5 in the viewpoint of pbp phylogeny. Three reference strains, 2603V/R (ATCC BAA-611, GenBank accession number NC004116), NEM316 (ATCC 12403, GenBank accession number NC004368), and COH1 (GenBank accession number AAJR01000000) are included. Bootstrap support values (500 replicates) are shown as percentages. Scale bars indicate the expected number of changes per sequence position.