TABLE 1.
Improved recovery of plasma HIV-1 RNA using a new extraction method
Sample | Extraction method | No. of replicates | Mean no. of HIV-1 RNA copies/ml detected | Mean % of expected HIV-1 RNA detecteda |
---|---|---|---|---|
Viremic plasma diluted 1:100 | v1.0 | 4 | 98 | 36 |
v2.0 | 4 | 292 | 108 | |
Viremic plasma diluted 1:1,000 | v1.0 | 6 | 12 | 44 |
v2.0 | 8 | 23 | 85 |
The numbers of expected HIV-1 RNA copies/ml detected were 271 for viremic plasma diluted 1:100 and 27.1 for viremic plasma diluted 1:1,000.