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. 2014 Nov;52(11):3890–3897. doi: 10.1128/JCM.01609-14

FIG 3.

FIG 3

Gel electrophoresis of the PCR targeting the Kingella kingae DNA uptake sequence. (A) One K. kingae strain was used for each of 9 studied sequence type complexes (STcs). Lane 1, STc-1; lane 2, STc-3; lane 3, STc-35; lane 4, STc-6; lane 5, STc-14; lane 6, STc-34; lane 7, STc-23; lane 8, STc-25; lane 9, STc-11; lanes L, DNA ladders. (B) Independent experiments using DNA extract of K. kingae type strain ATCC 23330 in quadruplicate using the same DNA extract (lanes 1 to 4) and with DNA derived from two different subcultures (lanes 5 and 6). Lanes L, DNA ladders. (C) Different dilutions of the DNA extract of K. kingae type strain ATCC 23330. Lane 1, pure; lane 2, 1/4; lane 3, 1/16; lane 4, 1/64; lane L, DNA ladders.