FIG 2.

Effect of the alignment strategy on classification of 16S ribosomal reads. Consensus reads, built from multiple subreads of the sequencing loop, were aligned to the LTP111 16S bacterial reference library using either PathoScope (x axis) or SMRT Portal (y axis). Each data point is a single patient's lung aspirate DNA sequences processed as described in Fig. 1. For each alignment strategy, the number of bacterial species utilizing >1% of the total bacterial reads was plotted.