FIG 5.

Typical taxonomic classification of bacteria in a lung aspirate. The pulmonary aspirate from a female ICU patient was characterized by clinical criteria (top) and by next-generation sequencing (NGS) of 16S amplimers from the genomic DNA (Bottom Panels). While the clinical and NGS diagnosis both include “Enterobacter” as a pathogen, the NGS diagnosis detects significant quantities of Raoultella, Citrobacter, and Klebsiella. Y.O., years old; F, female; WBC, white blood cells; PNM, polymorphonuclear; GNR, Gram-negative rod; OP, oral and oropharynx; Ab, antibiotic.