TABLE 1.
Characteristics of the in-house and commercial real-time PCR assays investigated
| Assay | LightCycler instrument | Target | Internal control | Sample vol/test (μl) | Reagent mix | Sensitivity |
|---|---|---|---|---|---|---|
| In-house | 1.5 | repMP1 copies | No | 5 | LC FastStart DNA Master HybProbe (Roche, Mannheim, Germany) | 0.2 CFUa |
| Diagenode | 2.0 | P1 adhesin | Yes | 10 | LC TaqMan Master (Roche) | 50 CCUb |
| GeneProof | 2.0 | CARDS toxinc | Yes | 10 | Included | No information |
| Ingenetix | 1.5 | 16S rRNA | Yes | 5 | LC FastStart DNA Master HybProbe | 0.9 CFUb |
| LightMix | 1.5 | P1 adhesin | Yes | 5 | LC FastStart DNA Master HybProbe | 10 copiesb |
a
According to Dumke et al. (11).
b
As specified by the manufacturer.
c
CARDS toxin, community-acquired respiratory distress syndrome toxin.