TABLE 3.

Results of four commercial real-time PCR assays used for investigation of 37 M. pneumoniae-positive respiratory tract samples compared to the in-house real-time PCR assay

Assay	No. of positive samples (n = 37) (%)	Crossing threshold of positive samples		95% CIa	P valueb
		Mean ± SD	Range of means
In-house	37 (100)	30.80 ± 2.72	23.29–35.75	29.92–31.68
Diagenode	37 (100)	31.50 ± 3.28	23.29–39.31	30.44–32.56	ns
GeneProof	34 (91.9)	30.80 ± 1.98	24.44–33.72	30.13–31.47	ns
Ingenetix	35 (94.6)	34.13 ± 2.94	26.41–40.85	33.16–35.10	<0.001
LightMix	35 (94.6)	32.29 ± 3.05	24.32–37.86	31.28–33.30	<0.001

^a95% CI, 95% confidence interval.

^bCalculated by paired Student's t test (only results of samples positive in both in-house and commercial assay were compared). ns, not significant.