TABLE 1.

Performance of PCR detection of Toxoplasma gondii in blood and ocular samples, calculation of Goldmann-Witmer coefficient, and immunoblotting for diagnosis of ocular toxoplasmosis^a

Sample	Method (nb)	Immune statusc	Group	No. of samples with result		% sensitivity	% specificity	Positive predictive value (%)	Negative predictive value (%)	P valued
				Positive	Negative
Blood samples	SV-PCR (405)	All patients	OT	5	116	4.1	99.6	83.3	70.9
			Control	1	283
		Immunocompetent	OT	1	69	1.4	99.4	50	70.5
			Control	1	165
		Immunocompromised	OT	4	20	16.7	100	100	67.7
			Control	0	42					<0.005
	LV-PCR (55)	All patients	OT	9	27	25	89.5	Not applicable	Not applicable
			Control	2	17
		Immunocompetent	OT	4	21	16	100	Not applicable	Not applicable
			Control	0	12
		Immunocompromised	OT	5	6	45.4	71.4	Not applicable	Not applicable
			Control	2	5					0.06
Ocular samples	PCR (434)	All patients	OT	47	84	35.9	100	100	78.3
			Control	0	303
		Immunocompetent	OT	18	56	24.3	100	100	74.3
			Control	0	162
		Immunocompromised	OT	16	10	61.5	100	100	81.5
			Control	0	44					<0.001
	Immunoblotting (363)	All patients	OT	59	65	47.6	100	100	78.6
			Control	0	239
		Immunocompetent	OT	33	39	45.8	100	100	76.9
			Control	0	130
		Immunocompromised	OT	9	12	42.9	100	100	74.5
			Control	0	35					0.88
	GWC (267)	All patients	OT	68	26	72.3	98.6	98.6	73.5
			Control	1	172
		Immunocompetent	OT	35	14	71.4	99	97.2	87.6
			Control	1	99
		Immunocompromised	OT	12	5	70.6	100	100	82.8
			Control	0	24					0.88

^aSV-PCR, small-volume PCR; LV-PCR, large-volume PCR; OT, ocular toxoplasmosis; GWC, calculation of the Goldmann-Witmer coefficient.

^bn, no. of samples.

^cPatients for whom immune status was available were categorized as “immunocompetent” or “immunocompromised.” Global analysis independent of the immune status is indicated on the “All patients” line.

^dAs calculated by the chi-square test between immunocompetent and immunocompromised groups.