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Canine fibroblast-like A-72 cells, 24 h postinfection, with puppy 4b CCoV. Phase-contrast microscopy of uninfected cell culture monolayer (A) and CCoV-infected cell culture monolayer with cytopathic effect characterized by rounding and death of individual cells (B) (10× original magnification). Immunofluorescence microscopy of CCoV-infected cell culture monolayer (mouse monoclonal antibody FIPV3-70 followed by Alexa Fluor 488-conjugated anti-IgG; 20× original magnification).
