TABLE 2.
Number of CFU per plate obtained from Helicobacter pyloria
| Dilution of starting bacterial suspensions (10 μl) (OD600, ca. 0.2) | No. of CFU per plate at the indicated no. of days of storage at 4°C |
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 |
1 |
2 |
3 |
4 |
5 |
7 |
10 |
|||||||||
| R | C | R | C | R | C | R | C | R | C | R | C | R | C | R | C | |
| 10 | >103 | >103 | >103 | >103 | >103 | >103 | >103 | >103 | >103 | >103 | >103 | 324 ± 35 | 150 ± 25 | 40 ± 7 | 4 ± 3 | 3 |
| 10−1 | >103 | >103 | >103 | 705 ± 90 | >103 | 208 ± 30 | >103 | 155 ± 25 | 321 ± 35 | 98 ± 20 | 375 ± 40 | 8 ± 3 | 3 | 0 | 2 | 1 |
| 10−2 | 490 ± 70 | 106 ± 15 | 450 ± 60 | 52 ± 20 | 471 ± 70 | 44 ± 8 | 420 ± 45 | 6 ± 2 | 88 ± 15 | 1 | 39 ± 8 | 0 | 0 | 0 | 0 | 0 |
| 10−3 | 170 ± 30 | 12 ± 6 | 165 ± 30 | 8 ± 4 | 158 ± 25 | 3 | 33 ± 8 | 0 | 7 ± 1 | 0 | 5 ± 3 | 0 | 0 | 0 | 0 | 0 |
| 10−4 | 9 ± 2 | 2 | 32 ± 7 | 2 | 1 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
a
R, reference strain H. pylori ATCC 43629; C, clinical strain H. pylori 13 A. Data represent the results determined with suspensions after storage at 4°C in GESA transport medium over time. The number of CFU per plate are mean values of the results of three experiments performed in duplicate ± standard deviations (SD).