FIG 2.

Phylogenetic relationship of the novel NTM isolate (TBL 1200985) and related species of Mycobacterium based on 16S rRNA gene sequences. All sequences used, except for the novel NTM, are type strains. Sequences were aligned with MUSCLE (17) and the tree constructed by the neighbor-joining method using the Tamura-Nei distance model. All steps were performed using Geneious software. Mycobacterium abscessus CIP 104536 was used as an outgroup (GenBank accession no. AY457071). Branch support is recorded at the nodes as a percentage of 100 bootstrap iterations.