FIG 4.
Phylogenetic relationship of the novel NTM isolate (TBL 1200985) and related species of Mycobacterium, based on the concatenation of hsp65, rpoB, and secA1 sequences. All sequences used, except for that of the novel NTM, are type strains. Sequences were aligned with MUSCLE (17) and the tree constructed by the neighbor-joining method using the Tamura-Nei distance model. All steps were performed using Geneious software. Nocardia farcinica ATCC 3318T was used as an outgroup (GenBank accession no. AY756523, AB243742, and AY781800). Branch support is recorded at the nodes as a percentage of 100 bootstrap iterations.